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cryopreserved hdfs (ATCC)

Name: cryopreserved hdfs
Brand: ATCC
Rating: 99 (2043 reviews)

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ATCC cryopreserved hdfs

Immunofluorescence intensity analysis for COL I, III, IV, V, VI, COL I/COL III ratio, TNMD and aSMA in hTCs and <t>hDFs</t> after 4, 7 and 10 days of culture under PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. (∗p < 0.05 vs respective PLLA group at the same time point; ‡p < 0.05 vs respective + TGFB2 group at the same time point; #p < 0.05 vs. respective + MMC group at the same time point; §p < 0.05 between cell types at the same time point and condition; &p < 0.05 vs. respective condition at day 4). N = 3.

Cryopreserved Hdfs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2043 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cryopreserved hdfs - by Bioz Stars, 2026-03

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1) Product Images from "Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage"

Article Title: Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

Journal: Bioactive Materials

doi: 10.1016/j.bioactmat.2025.11.008

Immunofluorescence intensity analysis for COL I, III, IV, V, VI, COL I/COL III ratio, TNMD and aSMA in hTCs and hDFs after 4, 7 and 10 days of culture under PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. (∗p < 0.05 vs respective PLLA group at the same time point; ‡p < 0.05 vs respective + TGFB2 group at the same time point; #p < 0.05 vs. respective + MMC group at the same time point; §p < 0.05 between cell types at the same time point and condition; &p < 0.05 vs. respective condition at day 4). N = 3.

Figure Legend Snippet: Immunofluorescence intensity analysis for COL I, III, IV, V, VI, COL I/COL III ratio, TNMD and aSMA in hTCs and hDFs after 4, 7 and 10 days of culture under PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. (∗p < 0.05 vs respective PLLA group at the same time point; ‡p < 0.05 vs respective + TGFB2 group at the same time point; #p < 0.05 vs. respective + MMC group at the same time point; §p < 0.05 between cell types at the same time point and condition; &p < 0.05 vs. respective condition at day 4). N = 3.

Techniques Used: Immunofluorescence

Principal component analysis ( A ) of the quantitative proteomic data obtained from hTCs and hDFs after 10 days of culture under TCP, PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions, as well as from TT samples. Venn diagram representing the top 100 protein hits in TT group vs. hTCs ( B ) and hDFs ( C ) cultured under PS, PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. Gene ontology analysis ( D ) of the top 100 protein hits in TT group vs. hTCs and hDFs cultured under PS, PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. N = 3.

Figure Legend Snippet: Principal component analysis ( A ) of the quantitative proteomic data obtained from hTCs and hDFs after 10 days of culture under TCP, PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions, as well as from TT samples. Venn diagram representing the top 100 protein hits in TT group vs. hTCs ( B ) and hDFs ( C ) cultured under PS, PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. Gene ontology analysis ( D ) of the top 100 protein hits in TT group vs. hTCs and hDFs cultured under PS, PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. N = 3.

Techniques Used: Cell Culture

Figure Legend Snippet: Gene set enrichment analysis of the quantitative proteomic profile of hDFs cultured for 10 days under PLLA ( A ), +TGFB2 ( B ), +MMC ( C ), and +MMC + TGFB2 ( D ) conditions. All contrasts were performed against hDFs cultured for 10 days under TCP conditions. A gene set containing all protein hits in TT group vs hDFs TCP group (tendon signature) was used for all analyses. Venn diagram summarizing the different core enrichment proteins identified in PLLA, +TGFB2, +MMC and +MMC + TGFB2 groups by means of gene set enrichment analysis ( E ). GO analysis of the core enrichment proteins identified in +MMC + TGFB2 group by means of gene set enrichment analysis ( F ). (NES: normalised enrichment score). N = 3.

Techniques Used: Cell Culture

Heatmap displaying the quantitative matrisome of hTCs and hDFs cultured under PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions for 10 days. Hierarchical clustering was applied to all samples. The heatmap displays the z-scores for every matrisome protein identified, being these grouped into the different matrisome categories. Both hTCs and hDFs clustered first in relation to TGFB2 treatment (alone or in combination with MMC), and ultimately in relation with the experimental conditions to which these were subjected. The + MMC + TGFB2 experimental condition exerted the highest changes into the quantitative matrisome of hTCs and hDFs, increasing ECM protein levels throughout all the categories of the matrisome. N = 3.

Figure Legend Snippet: Heatmap displaying the quantitative matrisome of hTCs and hDFs cultured under PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions for 10 days. Hierarchical clustering was applied to all samples. The heatmap displays the z-scores for every matrisome protein identified, being these grouped into the different matrisome categories. Both hTCs and hDFs clustered first in relation to TGFB2 treatment (alone or in combination with MMC), and ultimately in relation with the experimental conditions to which these were subjected. The + MMC + TGFB2 experimental condition exerted the highest changes into the quantitative matrisome of hTCs and hDFs, increasing ECM protein levels throughout all the categories of the matrisome. N = 3.

Techniques Used: Cell Culture

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Journal: Bioactive Materials

Article Title: Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

doi: 10.1016/j.bioactmat.2025.11.008

Figure Lengend Snippet: Immunofluorescence intensity analysis for COL I, III, IV, V, VI, COL I/COL III ratio, TNMD and aSMA in hTCs and hDFs after 4, 7 and 10 days of culture under PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. (∗p < 0.05 vs respective PLLA group at the same time point; ‡p < 0.05 vs respective + TGFB2 group at the same time point; #p < 0.05 vs. respective + MMC group at the same time point; §p < 0.05 between cell types at the same time point and condition; &p < 0.05 vs. respective condition at day 4). N = 3.

Article Snippet: Cryopreserved hDFs (PCS-201-012) were purchased from the American Type Culture Collection (ATCC, United Kingdom).

Techniques: Immunofluorescence

Journal: Bioactive Materials

Article Title: Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

doi: 10.1016/j.bioactmat.2025.11.008

Figure Lengend Snippet: Principal component analysis ( A ) of the quantitative proteomic data obtained from hTCs and hDFs after 10 days of culture under TCP, PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions, as well as from TT samples. Venn diagram representing the top 100 protein hits in TT group vs. hTCs ( B ) and hDFs ( C ) cultured under PS, PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. Gene ontology analysis ( D ) of the top 100 protein hits in TT group vs. hTCs and hDFs cultured under PS, PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions. N = 3.

Article Snippet: Cryopreserved hDFs (PCS-201-012) were purchased from the American Type Culture Collection (ATCC, United Kingdom).

Techniques: Cell Culture

Journal: Bioactive Materials

Article Title: Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

doi: 10.1016/j.bioactmat.2025.11.008

Figure Lengend Snippet: Gene set enrichment analysis of the quantitative proteomic profile of hDFs cultured for 10 days under PLLA ( A ), +TGFB2 ( B ), +MMC ( C ), and +MMC + TGFB2 ( D ) conditions. All contrasts were performed against hDFs cultured for 10 days under TCP conditions. A gene set containing all protein hits in TT group vs hDFs TCP group (tendon signature) was used for all analyses. Venn diagram summarizing the different core enrichment proteins identified in PLLA, +TGFB2, +MMC and +MMC + TGFB2 groups by means of gene set enrichment analysis ( E ). GO analysis of the core enrichment proteins identified in +MMC + TGFB2 group by means of gene set enrichment analysis ( F ). (NES: normalised enrichment score). N = 3.

Article Snippet: Cryopreserved hDFs (PCS-201-012) were purchased from the American Type Culture Collection (ATCC, United Kingdom).

Techniques: Cell Culture

Journal: Bioactive Materials

Article Title: Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

doi: 10.1016/j.bioactmat.2025.11.008

Figure Lengend Snippet: Heatmap displaying the quantitative matrisome of hTCs and hDFs cultured under PLLA, +TGFB2, +MMC and +MMC + TGFB2 conditions for 10 days. Hierarchical clustering was applied to all samples. The heatmap displays the z-scores for every matrisome protein identified, being these grouped into the different matrisome categories. Both hTCs and hDFs clustered first in relation to TGFB2 treatment (alone or in combination with MMC), and ultimately in relation with the experimental conditions to which these were subjected. The + MMC + TGFB2 experimental condition exerted the highest changes into the quantitative matrisome of hTCs and hDFs, increasing ECM protein levels throughout all the categories of the matrisome. N = 3.

Article Snippet: Cryopreserved hDFs (PCS-201-012) were purchased from the American Type Culture Collection (ATCC, United Kingdom).

Techniques: Cell Culture

Effects of liposome-encapsulated C.asiatica extract on fibroblast viability and migration. (A) Cell viability of normal human dermal fibroblasts (NHDFs) treated with EE, blank liposome, LEC, or vitamin E at concentrations of 6.5–100 µg/mL, as determined by the MTT assay. (B) Fibroblast migration assessed by scratch-wound assay following treatment with the same formulations and concentrations. Results are expressed as relative values normalized to the untreated control (set to 100%) and presented as mean ± SD ( n = 3). Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple-comparison test. Different letters above bars indicate statistically significant differences between treatment groups at the same concentration ( p < 0.05 ).

Journal: Frontiers in Medical Technology

Article Title: Evaluation of liposome - encapsulated Centella asiatica ethanolic extract for enhanced in vitro and in vivo wound healing

doi: 10.3389/fmedt.2026.1740835

Figure Lengend Snippet: Effects of liposome-encapsulated C.asiatica extract on fibroblast viability and migration. (A) Cell viability of normal human dermal fibroblasts (NHDFs) treated with EE, blank liposome, LEC, or vitamin E at concentrations of 6.5–100 µg/mL, as determined by the MTT assay. (B) Fibroblast migration assessed by scratch-wound assay following treatment with the same formulations and concentrations. Results are expressed as relative values normalized to the untreated control (set to 100%) and presented as mean ± SD ( n = 3). Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple-comparison test. Different letters above bars indicate statistically significant differences between treatment groups at the same concentration ( p < 0.05 ).

Article Snippet: Mouse macrophage cells (RAW 264.7, ATCC TIB-71) were used for anti-inflammatory assays, while normal human dermal fibroblasts (NHDF; ATCC PCS-201-012TM) were employed to model skin wound healing.

Techniques: Migration, MTT Assay, Scratch Wound Assay Assay, Control, Comparison, Concentration Assay

Representative hematoxylin and eosin (H&E)–stained sections of excision wound tissues on Day 12. ( A ) Liposome-encapsulated C.asiatica extract (LEC)–treated wounds, ( B ) vitamin E–treated wounds, ( C ) blank liposome control group, and ( D ) normal saline group. Black arrows indicate re-epithelialization and restoration of epidermal continuity, yellow arrows indicate fibroblast proliferation and granulation tissue formation, and red arrows indicate neovascularization. The LEC-treated group demonstrates complete re-epithelialization, dense fibroblast proliferation, and enhanced neovascularization compared with control groups. Scale bar = 400 µM.

Journal: Frontiers in Medical Technology

Article Title: Evaluation of liposome - encapsulated Centella asiatica ethanolic extract for enhanced in vitro and in vivo wound healing

doi: 10.3389/fmedt.2026.1740835

Figure Lengend Snippet: Representative hematoxylin and eosin (H&E)–stained sections of excision wound tissues on Day 12. ( A ) Liposome-encapsulated C.asiatica extract (LEC)–treated wounds, ( B ) vitamin E–treated wounds, ( C ) blank liposome control group, and ( D ) normal saline group. Black arrows indicate re-epithelialization and restoration of epidermal continuity, yellow arrows indicate fibroblast proliferation and granulation tissue formation, and red arrows indicate neovascularization. The LEC-treated group demonstrates complete re-epithelialization, dense fibroblast proliferation, and enhanced neovascularization compared with control groups. Scale bar = 400 µM.

Techniques: Staining, Control, Saline

$Histological evaluation and collagen deposition in excision wounds on Day 12. ( A ) Collagen content area (%) quantified from Masson's trichrome–stained sections in wounds treated with normal saline (NS), blank liposome ( B ), liposome-encapsulated C. asiatica extract (LEC), or vitamin E (VE) ( B ) Semi-quantitative histological scores for re-epithelialization, fibroblast/granulation tissue formation, and neovascularization, assessed using a standardized ordinal scale ranging from 0 (absent) to 3 (marked) Data are presented as mean ± SD ( n = 5 rats per group). Statistical analysis for collagen area fraction was performed using one-way ANOVA followed by Tukey's multiple-comparison test, while histological scores were analyzed using the Kruskal–Wallis test followed by Dunn's multiple-comparison test. Different letters above bars indicate statistically significant differences among treatment groups within the same parameter ( p < 0.05).$

Journal: Frontiers in Medical Technology

Article Title: Evaluation of liposome - encapsulated Centella asiatica ethanolic extract for enhanced in vitro and in vivo wound healing

doi: 10.3389/fmedt.2026.1740835

Figure Lengend Snippet: Histological evaluation and collagen deposition in excision wounds on Day 12. ( A ) Collagen content area (%) quantified from Masson's trichrome–stained sections in wounds treated with normal saline (NS), blank liposome ( B ), liposome-encapsulated C. asiatica extract (LEC), or vitamin E (VE) ( B ) Semi-quantitative histological scores for re-epithelialization, fibroblast/granulation tissue formation, and neovascularization, assessed using a standardized ordinal scale ranging from 0 (absent) to 3 (marked) Data are presented as mean ± SD ( n = 5 rats per group). Statistical analysis for collagen area fraction was performed using one-way ANOVA followed by Tukey's multiple-comparison test, while histological scores were analyzed using the Kruskal–Wallis test followed by Dunn's multiple-comparison test. Different letters above bars indicate statistically significant differences among treatment groups within the same parameter ( p < 0.05).

Techniques: Staining, Saline, Comparison

a – c Intracellular calcium levels in response to treatment with 225 in different cellular compartments: mitochondria ( a ), ER ( b ) and cytosol ( c ). Sensitive cell lines (MCF7ca, A375, A253) exhibited significantly increased calcium levels, while insensitive cell lines (MDA-MB-231, MDA-MB-453, T-47D) and human normal fibroblasts (HDFa, GM05294) showed no significant changes. Data are presented as mean ± SD. n = 4 biological replicates. Statistical significance was determined by unpaired two-tailed Student’s t-test. **** p < 0.0001, *** p < 0.001, ** p < 0.01. ns means not significant. d VDAC1 expression levels in representative 225-sensitive, 225-insensitive, and normal cell lines, assessed by Western blot (WB). Data shown are representative of three independent experiments. e Quantification of VDAC1 protein levels normalized to β-actin for the samples shown in ( d ).

Journal: Nature Communications

Article Title: A small molecule VDAC ligand inhibits ERAD and induces selective cancer cell death via disruption of calcium homeostasis

doi: 10.1038/s41467-025-67816-z

Figure Lengend Snippet: a – c Intracellular calcium levels in response to treatment with 225 in different cellular compartments: mitochondria ( a ), ER ( b ) and cytosol ( c ). Sensitive cell lines (MCF7ca, A375, A253) exhibited significantly increased calcium levels, while insensitive cell lines (MDA-MB-231, MDA-MB-453, T-47D) and human normal fibroblasts (HDFa, GM05294) showed no significant changes. Data are presented as mean ± SD. n = 4 biological replicates. Statistical significance was determined by unpaired two-tailed Student’s t-test. **** p < 0.0001, *** p < 0.001, ** p < 0.01. ns means not significant. d VDAC1 expression levels in representative 225-sensitive, 225-insensitive, and normal cell lines, assessed by Western blot (WB). Data shown are representative of three independent experiments. e Quantification of VDAC1 protein levels normalized to β-actin for the samples shown in ( d ).

Article Snippet: HeLa (CCL-2), HepG2 (HB-8065), A-375 (CRL-1619), U-2OS (HTB-96), A253 (HTB-41), PSN1 (CRL-3211), Primary Dermal Fibroblast (HDFa, PCS-201-012) and Primary Epidermal Melanocyte (HEMa, PCS-200-013) cell lines were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Two Tailed Test, Expressing, Western Blot

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